Dataset Information

Colonic patch and colonic SILT development are independent and differentially regulated events.

ABSTRACT: Intestinal lymphoid tissues have to simultaneously ensure protection against pathogens and tolerance toward commensals. Despite such vital functions, their development in the colon is poorly understood. Here, we show that the two distinct lymphoid tissues of the colon-colonic patches and colonic solitary intestinal lymphoid tissues (SILTs)-can easily be distinguished based on anatomical location, developmental timeframe, and cellular organization. Furthermore, whereas colonic patch development depended on CXCL13-mediated lymphoid tissue inducer (LTi) cell clustering followed by LT?-mediated consolidation, early LTi clustering at SILT anlagen did not require CXCL13, CCR6, or CXCR3. Subsequent dendritic cell recruitment to and gp38(+)VCAM-1(+) lymphoid stromal cell differentiation within SILTs required LT?; B-cell recruitment and follicular dendritic cell differentiation depended on MyD88-mediated signaling, but not the microflora. In conclusion, our data demonstrate that different mechanisms, mediated mainly by programmed stimuli, induce the formation of distinct colonic lymphoid tissues, therefore suggesting that these tissues may have different functions.

SUBMITTER: Baptista AP

PROVIDER: S-EPMC3570605 | biostudies-literature | 2013 May

REPOSITORIES: biostudies-literature

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Colonic patch and colonic SILT development are independent and differentially regulated events.

Baptista A P AP Olivier B J BJ Goverse G G Greuter M M Knippenberg M M Kusser K K Domingues R G RG Veiga-Fernandes H H Luster A D AD Lugering A A Randall T D TD Cupedo T T Mebius R E RE

Mucosal immunology 20120919 3

Intestinal lymphoid tissues have to simultaneously ensure protection against pathogens and tolerance toward commensals. Despite such vital functions, their development in the colon is poorly understood. Here, we show that the two distinct lymphoid tissues of the colon-colonic patches and colonic solitary intestinal lymphoid tissues (SILTs)-can easily be distinguished based on anatomical location, developmental timeframe, and cellular organization. Furthermore, whereas colonic patch development d ...[more]

PMID: 22990625

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Dataset Information

Colonic patch and colonic SILT development are independent and differentially regulated events.

Publications

Colonic patch and colonic SILT development are independent and differentially regulated events.

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