Ontology highlight
ABSTRACT:
SUBMITTER: Wang W
PROVIDER: S-EPMC3570649 | biostudies-literature | 2013 Feb
REPOSITORIES: biostudies-literature
Structure (London, England : 1993) 20121227 2
The essential splicing factors U2AF⁶⁵ and SF1 cooperatively bind consensus sequences at the 3' end of introns. Phosphorylation of SF1 on a highly conserved "SPSP" motif enhances its interaction with U2AF⁶⁵ and the pre-mRNA. Here, we reveal that phosphorylation induces essential conformational changes in SF1 and in the SF1/U2AF⁶⁵/3' splice site complex. Crystal structures of the phosphorylated (P)SF1 domain bound to the C-terminal domain of U2AF⁶⁵ at 2.29 Å resolution and of the unphosphorylated ...[more]