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Single-molecule investigation of G-quadruplex using a nanopore sensor.

ABSTRACT: This review article introduces the nanopore single-molecule method for the study of G-quadruplex nucleic acid structures. Single G-quadruplexes can be trapped into a 2 nm protein pore embedded in the lipid bilayer membrane. The trapped G-quadruplex specifically blocks the current through the nanopore, creating a signature event for quantitative analysis of G-quadruplex properties, from cation-determined folding and unfolding kinetics to the interactions with the protein ligand. The nanopore single-molecule method is simple, accurate, and requires no labels. It can be used to evaluate G-quadruplex mechanisms and it may have applications in G-quadruplex-based biosensors, nanomachines, and nanostructure assembly.

SUBMITTER: Shim J

PROVIDER: S-EPMC3572831 | biostudies-literature | 2012 May

REPOSITORIES: biostudies-literature

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Single-molecule investigation of G-quadruplex using a nanopore sensor.

Shim Jiwook J Gu Li-Qun LQ

Methods (San Diego, Calif.) 20120402 1

This review article introduces the nanopore single-molecule method for the study of G-quadruplex nucleic acid structures. Single G-quadruplexes can be trapped into a 2 nm protein pore embedded in the lipid bilayer membrane. The trapped G-quadruplex specifically blocks the current through the nanopore, creating a signature event for quantitative analysis of G-quadruplex properties, from cation-determined folding and unfolding kinetics to the interactions with the protein ligand. The nanopore sing ...[more]

PMID: 22487183

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