Subangstrom single-molecule measurements of motor proteins using a nanopore.
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ABSTRACT:
SUBMITTER: Derrington IM
PROVIDER: S-EPMC4915380 | biostudies-literature | 2015 Oct
REPOSITORIES: biostudies-literature
Publications
Subangstrom single-molecule measurements of motor proteins using a nanopore.
Derrington Ian M IM Craig Jonathan M JM Stava Eric E Laszlo Andrew H AH Ross Brian C BC Brinkerhoff Henry H Nova Ian C IC Doering Kenji K Tickman Benjamin I BI Ronaghi Mostafa M Mandell Jeffrey G JG Gunderson Kevin L KL Gundlach Jens H JH
Nature biotechnology 20150928 10
Techniques for measuring the motion of single motor proteins, such as FRET and optical tweezers, are limited to a resolution of ∼300 pm. We use ion current modulation through the protein nanopore MspA to observe translocation of helicase Hel308 on DNA with up to ∼40 pm sensitivity. This approach should be applicable to any protein that translocates on DNA or RNA, including helicases, polymerases, recombinases and DNA repair enzymes. ...[more]
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