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Subangstrom single-molecule measurements of motor proteins using a nanopore.

ABSTRACT: Techniques for measuring the motion of single motor proteins, such as FRET and optical tweezers, are limited to a resolution of ?300 pm. We use ion current modulation through the protein nanopore MspA to observe translocation of helicase Hel308 on DNA with up to ?40 pm sensitivity. This approach should be applicable to any protein that translocates on DNA or RNA, including helicases, polymerases, recombinases and DNA repair enzymes.

SUBMITTER: Derrington IM 

PROVIDER: S-EPMC4915380 | biostudies-literature | 2015 Oct

REPOSITORIES: biostudies-literature

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Techniques for measuring the motion of single motor proteins, such as FRET and optical tweezers, are limited to a resolution of ∼300 pm. We use ion current modulation through the protein nanopore MspA to observe translocation of helicase Hel308 on DNA with up to ∼40 pm sensitivity. This approach should be applicable to any protein that translocates on DNA or RNA, including helicases, polymerases, recombinases and DNA repair enzymes. ...[more]

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