Ontology highlight
ABSTRACT: Background
Gene-based vaccination using prime/boost regimens protects animals and humans against malaria, inducing cell-mediated responses that in animal models target liver stage malaria parasites. We tested a DNA prime/adenovirus boost malaria vaccine in a Phase 1 clinical trial with controlled human malaria infection.Methodology/principal findings
The vaccine regimen was three monthly doses of two DNA plasmids (DNA) followed four months later by a single boost with two non-replicating human serotype 5 adenovirus vectors (Ad). The constructs encoded genes expressing P. falciparum circumsporozoite protein (CSP) and apical membrane antigen-1 (AMA1). The regimen was safe and well-tolerated, with mostly mild adverse events that occurred at the site of injection. Only one AE (diarrhea), possibly related to immunization, was severe (Grade 3), preventing daily activities. Four weeks after the Ad boost, 15 study subjects were challenged with P. falciparum sporozoites by mosquito bite, and four (27%) were sterilely protected. Antibody responses by ELISA rose after Ad boost but were low (CSP geometric mean titer 210, range 44-817; AMA1 geometric mean micrograms/milliliter 11.9, range 1.5-102) and were not associated with protection. Ex vivo IFN-? ELISpot responses after Ad boost were modest (CSP geometric mean spot forming cells/million peripheral blood mononuclear cells 86, range 13-408; AMA1 348, range 88-1270) and were highest in three protected subjects. ELISpot responses to AMA1 were significantly associated with protection (p?=?0.019). Flow cytometry identified predominant IFN-? mono-secreting CD8+ T cell responses in three protected subjects. No subjects with high pre-existing anti-Ad5 neutralizing antibodies were protected but the association was not statistically significant.Significance
The DNA/Ad regimen provided the highest sterile immunity achieved against malaria following immunization with a gene-based subunit vaccine (27%). Protection was associated with cell-mediated immunity to AMA1, with CSP probably contributing. Substituting a low seroprevalence vector for Ad5 and supplementing CSP/AMA1 with additional antigens may improve protection.Trial registration
ClinicalTrials.govNCT00870987.
SUBMITTER: Chuang I
PROVIDER: S-EPMC3573028 | biostudies-literature | 2013
REPOSITORIES: biostudies-literature
Chuang Ilin I Sedegah Martha M Cicatelli Susan S Spring Michele M Polhemus Mark M Tamminga Cindy C Patterson Noelle N Guerrero Melanie M Bennett Jason W JW McGrath Shannon S Ganeshan Harini H Belmonte Maria M Farooq Fouzia F Abot Esteban E Banania Jo Glenna JG Huang Jun J Newcomer Rhonda R Rein Lisa L Litilit Dianne D Richie Nancy O NO Wood Chloe C Murphy Jittawadee J Sauerwein Robert R Hermsen Cornelus C CC McCoy Andrea J AJ Kamau Edwin E Cummings James J Komisar Jack J Sutamihardja Awalludin A Shi Meng M Epstein Judith E JE Maiolatesi Santina S Tosh Donna D Limbach Keith K Angov Evelina E Bergmann-Leitner Elke E Bruder Joseph T JT Doolan Denise L DL King C Richter CR Carucci Daniel D Dutta Sheetij S Soisson Lorraine L Diggs Carter C Hollingdale Michael R MR Ockenhouse Christian F CF Richie Thomas L TL
PloS one 20130214 2
<h4>Background</h4>Gene-based vaccination using prime/boost regimens protects animals and humans against malaria, inducing cell-mediated responses that in animal models target liver stage malaria parasites. We tested a DNA prime/adenovirus boost malaria vaccine in a Phase 1 clinical trial with controlled human malaria infection.<h4>Methodology/principal findings</h4>The vaccine regimen was three monthly doses of two DNA plasmids (DNA) followed four months later by a single boost with two non-rep ...[more]