Ontology highlight
ABSTRACT:
SUBMITTER: Katter K
PROVIDER: S-EPMC3574282 | biostudies-literature | 2013 Mar
REPOSITORIES: biostudies-literature
Katter Katharina K Geurts Aron M AM Hoffmann Orsolya O Mátés Lajos L Landa Vladimir V Hiripi László L Moreno Carol C Lazar Jozef J Bashir Sanum S Zidek Vaclav V Popova Elena E Jerchow Boris B Becker Katja K Devaraj Anantharam A Walter Ingrid I Grzybowksi Michael M Corbett Molly M Filho Artur Rangel AR Hodges Matthew R MR Bader Michael M Ivics Zoltán Z Jacob Howard J HJ Pravenec Michal M Bosze Zsuzsanna Z Rülicke Thomas T Izsvák Zsuzsanna Z
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 20121129 3
Germline transgenesis is an important procedure for functional investigation of biological pathways, as well as for animal biotechnology. We have established a simple, nonviral protocol in three important biomedical model organisms frequently used in physiological studies. The protocol is based on the hyperactive Sleeping Beauty transposon system, SB100X, which reproducibly promoted generation of transgenic founders at frequencies of 50-64, 14-72, and 15% in mice, rats, and rabbits, respectively ...[more]