RNA interference rescue by bacterial artificial chromosome transgenesis in mammalian tissue culture cells.
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ABSTRACT:
SUBMITTER: Kittler R
PROVIDER: S-EPMC548992 | biostudies-literature | 2005 Feb
REPOSITORIES: biostudies-literature
Publications
RNA interference rescue by bacterial artificial chromosome transgenesis in mammalian tissue culture cells.
Kittler Ralf R Pelletier Laurence L Ma Chunling C Poser Ina I Fischer Steffi S Hyman Anthony A AA Buchholz Frank F
Proceedings of the National Academy of Sciences of the United States of America 20050203 7
RNA interference (RNAi) is a widely used method for analysis of gene function in tissue culture cells. However, to date there has been no reliable method for testing the specificity of any particular RNAi experiment. The ideal experiment is to rescue the phenotype by expression of the target gene in a form refractory to RNAi. The transgene should be expressed at physiological levels and with its different splice variants. Here, we demonstrate that expression of murine bacterial artificial chromo ...[more]
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