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Engineering of interlocked DNA G-quadruplexes as a robust scaffold.

ABSTRACT: Interlock is a structural element in DNA G-quadruplexes that can be compared with the commonly used complementary binding of 'sticky ends' in DNA duplexes. G-quadruplex interlocking can be a basis for the assembly of higher-order structures. In this study, we formulated a rule to engineer (3 + 1) interlocked dimeric G-quadruplexes and established the folding topology of the designed DNA sequences by nuclear magnetic resonance spectroscopy. These interlocked G-quadruplexes are very stable and can serve as compact robust scaffolds for various applications. Different structural elements can be engineered in these robust scaffolds. We demonstrated the anti-HIV inhibition activity of the newly designed DNA sequences.

SUBMITTER: Phan AT

PROVIDER: S-EPMC3575790 | biostudies-literature | 2013 Feb

REPOSITORIES: biostudies-literature

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Engineering of interlocked DNA G-quadruplexes as a robust scaffold.

Phan Anh Tuân AT Do Ngoc Quang NQ

Nucleic acids research 20121228 4

Interlock is a structural element in DNA G-quadruplexes that can be compared with the commonly used complementary binding of 'sticky ends' in DNA duplexes. G-quadruplex interlocking can be a basis for the assembly of higher-order structures. In this study, we formulated a rule to engineer (3 + 1) interlocked dimeric G-quadruplexes and established the folding topology of the designed DNA sequences by nuclear magnetic resonance spectroscopy. These interlocked G-quadruplexes are very stable and can ...[more]

PMID: 23275539

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