Ontology highlight
ABSTRACT:
SUBMITTER: Freudiger CW
PROVIDER: S-EPMC3576036 | biostudies-literature | 2008 Dec
REPOSITORIES: biostudies-literature
Freudiger Christian W CW Min Wei W Saar Brian G BG Lu Sijia S Holtom Gary R GR He Chengwei C Tsai Jason C JC Kang Jing X JX Xie X Sunney XS
Science (New York, N.Y.) 20081201 5909
Label-free chemical contrast is highly desirable in biomedical imaging. Spontaneous Raman microscopy provides specific vibrational signatures of chemical bonds, but is often hindered by low sensitivity. Here we report a three-dimensional multiphoton vibrational imaging technique based on stimulated Raman scattering (SRS). The sensitivity of SRS imaging is significantly greater than that of spontaneous Raman microscopy, which is achieved by implementing high-frequency (megahertz) phase-sensitive ...[more]