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Label-free quantitative imaging of cholesterol in intact tissues by hyperspectral stimulated Raman scattering microscopy.


ABSTRACT: A finger on the pulse: Current molecular analysis of cells and tissues routinely relies on separation, enrichment, and subsequent measurements by various assays. Now, a platform of hyperspectral stimulated Raman scattering microscopy has been developed for the fast, quantitative, and label-free imaging of biomolecules in intact tissues using spectroscopic fingerprints as the contrast mechanism.

SUBMITTER: Wang P 

PROVIDER: S-EPMC3932421 | biostudies-literature | 2013 Dec

REPOSITORIES: biostudies-literature

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Label-free quantitative imaging of cholesterol in intact tissues by hyperspectral stimulated Raman scattering microscopy.

Wang Ping P   Li Junjie J   Wang Pu P   Hu Chun-Rui CR   Zhang Delong D   Sturek Michael M   Cheng Ji-Xin JX  

Angewandte Chemie (International ed. in English) 20131014 49


A finger on the pulse: Current molecular analysis of cells and tissues routinely relies on separation, enrichment, and subsequent measurements by various assays. Now, a platform of hyperspectral stimulated Raman scattering microscopy has been developed for the fast, quantitative, and label-free imaging of biomolecules in intact tissues using spectroscopic fingerprints as the contrast mechanism. ...[more]

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