Ontology highlight
ABSTRACT:
SUBMITTER: Masujin K
PROVIDER: S-EPMC3585212 | biostudies-literature | 2013
REPOSITORIES: biostudies-literature
Masujin Kentaro K Kaku-Ushiki Yuko Y Miwa Ritsuko R Okada Hiroyuki H Shimizu Yoshihisa Y Kasai Kazuo K Matsuura Yuichi Y Yokoyama Takashi T
PloS one 20130228 2
The conformation of abnormal prion protein (PrP(Sc)) differs from that of cellular prion protein (PrP(C)), but the precise characteristics of PrP(Sc) remain to be elucidated. To clarify the properties of native PrP(Sc), we attempted to generate novel PrP(Sc)-specific monoclonal antibodies (mAbs) by immunizing PrP-deficient mice with intact PrP(Sc) purified from bovine spongiform encephalopathy (BSE)-affected mice. The generated mAbs 6A12 and 8D5 selectivity precipitated PrP(Sc) from the brains o ...[more]