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Parallel in vivo DNA assembly by recombination: experimental demonstration and theoretical approaches.

ABSTRACT: The development of synthetic biology requires rapid batch construction of large gene networks from combinations of smaller units. Despite the availability of computational predictions for well-characterized enzymes, the optimization of most synthetic biology projects requires combinational constructions and tests. A new building-brick-style parallel DNA assembly framework for simple and flexible batch construction is presented here. It is based on robust recombination steps and allows a variety of DNA assembly techniques to be organized for complex constructions (with or without scars). The assembly of five DNA fragments into a host genome was performed as an experimental demonstration.

SUBMITTER: Shi Z

PROVIDER: S-EPMC3585241 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature

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Parallel in vivo DNA assembly by recombination: experimental demonstration and theoretical approaches.

Shi Zhenyu Z Wedd Anthony G AG Gras Sally L SL

PloS one 20130228 2

The development of synthetic biology requires rapid batch construction of large gene networks from combinations of smaller units. Despite the availability of computational predictions for well-characterized enzymes, the optimization of most synthetic biology projects requires combinational constructions and tests. A new building-brick-style parallel DNA assembly framework for simple and flexible batch construction is presented here. It is based on robust recombination steps and allows a variety ...[more]

PMID: 23468883

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