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Expression of ephrin receptors and ligands in postmortem brains of HIV-infected subjects with and without cognitive impairment.

ABSTRACT: Despite the successes of combination antiretroviral therapy, HIV-associated neurocognitive disorders persist in many infected individuals. Earlier studies showed that neurocognitive impairment was associated with glutamate toxicity and synaptodendritic damage. We examined alterations in expression of four ephrin genes that are involved in synapse formation and recruitment of glutamate receptors to synapses, in the caudate and anterior cingulate in postmortem brain of cognitively characterized HIV-infected subjects, along with expression of neuronal and astroglial/macroglial markers. Postmortem tissues of HIV-infected and control subjects were obtained from the Manhattan HIV Brain Bank. HIV-infected subjects underwent neurocognitive assessment prior to death. Quantification of mRNA of genes of chemokine receptors and chemokines (CCR5, CXCR4, CCL2), astroglial/microglial markers (GFAP, CD163, CD68), the neuronal marker SNAP25, ephrin receptors EPHA4 and EPHB2, and ephrin ligands EFNB1 and EFNB2 was performed using SYBR Green RT-PCR. Proinflammatory chemokine and glial/macrophage mRNA levels in both regions were significantly greater in HIV+ than in HIV- subjects. Levels of EPHA4 and EFNB2 mRNA in the caudate, and EPHB2 mRNA in anterior cingulate were significantly lower in HIV+ subjects (p?

SUBMITTER: Yuferov V

PROVIDER: S-EPMC3587720 | biostudies-literature | 2013 Mar

REPOSITORIES: biostudies-literature

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Expression of ephrin receptors and ligands in postmortem brains of HIV-infected subjects with and without cognitive impairment.

Yuferov Vadim V Ho Ann A Morgello Susan S Yang Yaning Y Ott Jurg J Kreek Mary Jeanne MJ

Journal of neuroimmune pharmacology : the official journal of the Society on NeuroImmune Pharmacology 20130112 1

Despite the successes of combination antiretroviral therapy, HIV-associated neurocognitive disorders persist in many infected individuals. Earlier studies showed that neurocognitive impairment was associated with glutamate toxicity and synaptodendritic damage. We examined alterations in expression of four ephrin genes that are involved in synapse formation and recruitment of glutamate receptors to synapses, in the caudate and anterior cingulate in postmortem brain of cognitively characterized HI ...[more]

PMID: 23314923

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Expression of ephrin receptors and ligands in postmortem brains of HIV-infected subjects with and without cognitive impairment.

Publications

Expression of ephrin receptors and ligands in postmortem brains of HIV-infected subjects with and without cognitive impairment.

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