Dataset Information

Complete Genome Sequence of the Industrial Strain Gluconobacter oxydans H24.

ABSTRACT: Gluconobacter oxydans is characterized by its ability to incompletely oxidize carbohydrates and alcohols. The high yields of its oxidation products and complete secretion into the medium make it important for industrial use. We report the finished genome sequence of Gluconobacter oxydans H24, an industrial strain with high l-sorbose productivity.

SUBMITTER: Ge X

PROVIDER: S-EPMC3587919 | biostudies-literature | 2013 Jan

REPOSITORIES: biostudies-literature

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Complete Genome Sequence of the Industrial Strain Gluconobacter oxydans H24.

Ge Xin X Zhao Yan Y Hou Wei W Zhang Weicai W Chen Weiwei W Wang Jianhua J Zhao Nan N Lin Jian J Wang Wenxi W Chen Mengxia M Wang Qingge Q Jiao Yinghui Y Yuan Zhigang Z Xiong Xianghua X

Genome announcements 20130101 1

<i>Gluconobacter oxydans</i> is characterized by its ability to incompletely oxidize carbohydrates and alcohols. The high yields of its oxidation products and complete secretion into the medium make it important for industrial use. We report the finished genome sequence of <i>Gluconobacter oxydans</i> H24, an industrial strain with high l-sorbose productivity. ...[more]

PMID: 23472221

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Project description:L-(+)-tartaric acid (L-TA) is an important organic acid, which is produced from the cream of tartar or stereospecific hydrolysis of the cis-epoxysuccinate. The former method is limited by the availability of raw material and the latter is dependent on the petrochemical material. Thus, new processes for the economical preparation of L-TA from carbohydrate or renewable resource would be much more attractive. Production of 5-keto-D-gluconate (5-KGA) from glucose by Gluconobacter oxydans is the first step to produce L-TA. The aim of this work is to enhance 5-KGA accumulation using combinatorial metabolic engineering strategies in G. oxydans. The sldAB gene, encoding sorbitol dehydrogenase, was overexpressed in an industrial strain G. oxydans ZJU2 under a carefully selected promoter, P0169. To enhance the efficiency of the oxidation by sldAB, the coenzyme pyrroloquinoline quinone (PQQ) and respiratory chain were engineered. Besides, the role in sldAB overexpression, coenzyme and respiratory chain engineering and their subsequent effects on 5-KGA production were investigated.An efficient, stable recombinant strain was constructed, whereas the 5-KGA production could be enhanced. By self-overexpressing the sldAB gene in G. oxydans ZJU2 under the constitutive promoter P0169, the resulting strain, G. oxydans ZJU3, produced 122.48?±?0.41 g/L of 5-KGA. Furthermore, through the coenzyme and respiratory chain engineering, the titer and productivity of 5-KGA reached 144.52?±?2.94 g/L and 2.26 g/(L?·?h), respectively, in a 15 L fermenter. It could be further improved the 5-KGA titer by 12.10 % through the fed-batch fermentation under the pH shift and dissolved oxygen tension (DOT) control condition, obtained 162?±?2.12 g/L with the productivity of 2.53 g/(L?·?h) within 64 h.The 5-KGA production could be significantly enhanced with the combinatorial metabolic engineering strategy in Gluconobacter strain, including sldAB overexpression, coenzyme and respiratory chain engineering. Fed-batch fermentation could further enlarge the positive effect and increase the 5-KGA production. All of these demonstrated that the robust recombinant strain can efficiently produce 5-KGA in larger scale to fulfill the industrial production of L-TA from 5-KGA.

Dataset Information

Complete Genome Sequence of the Industrial Strain Gluconobacter oxydans H24.

Publications

Complete Genome Sequence of the Industrial Strain Gluconobacter oxydans H24.

Similar Datasets

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