Dataset Information

Draft Genome Sequence of Gluconobacter oxydans NL71, a Strain That Efficiently Biocatalyzes Xylose to Xylonic Acid at a High Concentration.

ABSTRACT: Gluconobacter oxydans NL71, a selected strain in the crude lignocellulosic hydrolysate, catalyzed 600 g/liter xylose to 586.3 g/liter xylonic acid at 95.1% yield. The biocatalysis of xylose yielded three times higher than the best previous output, providing a possibility of the industrial scale utilization of lignocellulosic xylose. Due to its promising industrial applications, we sequenced the complete genome of strain G. oxydans NL71 to further our understanding of its overall metabolism.

SUBMITTER: Miao Y

PROVIDER: S-EPMC4472894 | biostudies-literature | 2015 Jun

REPOSITORIES: biostudies-literature

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Draft Genome Sequence of Gluconobacter oxydans NL71, a Strain That Efficiently Biocatalyzes Xylose to Xylonic Acid at a High Concentration.

Miao Yuanyuan Y Zhou Xin X Xu Yong Y Yu Shiyuan S

Genome announcements 20150618 3

Gluconobacter oxydans NL71, a selected strain in the crude lignocellulosic hydrolysate, catalyzed 600 g/liter xylose to 586.3 g/liter xylonic acid at 95.1% yield. The biocatalysis of xylose yielded three times higher than the best previous output, providing a possibility of the industrial scale utilization of lignocellulosic xylose. Due to its promising industrial applications, we sequenced the complete genome of strain G. oxydans NL71 to further our understanding of its overall metabolism. ...[more]

PMID: 26089417

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Project description:The acetic acid bacterium (AAB) Gluconobacter oxydans incompletely oxidizes a wide variety of carbohydrates and is therefore used industrially for oxidative biotransformations. For G. oxydans, no system was available that allows regulatable plasmid-based expression. We found that the L-arabinose-inducible PBAD promoter and the transcriptional regulator AraC from Escherichia coli MC4100 performed very well in G. oxydans. The respective pBBR1-based plasmids showed very low basal expression of the reporters β-glucuronidase and mNeonGreen, up to 480-fold induction with 1% L-arabinose, and tunability from 0.1 to 1% L-arabinose. In G. oxydans 621H, L-arabinose was oxidized by the membrane-bound glucose dehydrogenase, which is absent in the multi-deletion strain BP.6. Nevertheless, AraC-PBAD performed similar in both strains in the exponential phase, indicating that a gene knockout is not required for application of AraC-PBAD in wild-type G. oxydans strains. However, the oxidation product arabinonic acid strongly contributed to the acidification of the growth medium in 621H cultures during the stationary phase, which resulted in drastically decreased reporter activities in 621H (pH 3.3) but not in BP.6 cultures (pH 4.4). These activities could be strongly increased quickly solely by incubating stationary cells in D-mannitol-free medium adjusted to pH 6, indicating that the reporters were hardly degraded yet rather became inactive. In a pH-controlled bioreactor, these reporter activities remained high in the stationary phase (pH 6). Finally, we created a multiple cloning vector with araC-PBAD based on pBBR1MCS-5. Together, we demonstrated superior functionality and good tunability of an AraC-PBAD system in G. oxydans that could possibly also be used in other AAB. KEY POINTS: • We found the AraC-PBAD system from E. coli MC4100 was well tunable in G. oxydans. • In the absence of AraC or l-arabinose, expression from PBAD was extremely low. • This araC-PBAD system could also be fully functional in other acetic acid bacteria.

Dataset Information

Draft Genome Sequence of Gluconobacter oxydans NL71, a Strain That Efficiently Biocatalyzes Xylose to Xylonic Acid at a High Concentration.

Publications

Draft Genome Sequence of Gluconobacter oxydans NL71, a Strain That Efficiently Biocatalyzes Xylose to Xylonic Acid at a High Concentration.

Similar Datasets

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