In?Situ probing of newly synthesized peptidoglycan in live bacteria with fluorescent D-amino acids.
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ABSTRACT:
SUBMITTER: Kuru E
PROVIDER: S-EPMC3589519 | biostudies-literature | 2012 Dec
REPOSITORIES: biostudies-literature
Publications
In Situ probing of newly synthesized peptidoglycan in live bacteria with fluorescent D-amino acids.
Kuru Erkin E Hughes H Velocity HV Brown Pamela J PJ Hall Edward E Tekkam Srinivas S Cava Felipe F de Pedro Miguel A MA Brun Yves V YV VanNieuwenhze Michael S MS
Angewandte Chemie (International ed. in English) 20121010 50
Tracking a bug's life: Peptidoglycan (PG) of diverse bacteria is labeled by exploiting the tolerance of cells for incorporating different non-natural D-amino acids. These nontoxic D-amino acids preferably label the sites of active PG synthesis, thereby enabling fine spatiotemporal tracking of cell-wall dynamics in phylogenetically and morphologically diverse bacteria. HCC = 7-hydroxycoumarin, NBD = 7-nitrobenzofurazan, TAMRA = carboxytetramethylrhodamine. ...[more]
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