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Anatomical and transcriptional dynamics of maize embryonic leaves during seed germination.


ABSTRACT: Our anatomical analysis revealed that a dry maize seed contains four to five embryonic leaves at different developmental stages. Rudimentary kranz structure (KS) is apparent in the first leaf with a substantial density, but its density decreases toward younger leaves. Upon imbibition, leaf expansion occurs rapidly with new KSs initiated from the palisade-like ground meristem cells in the middle of the leaf. In parallel to the anatomical analysis, we obtained the time course transcriptomes for the embryonic leaves in dry and imbibed seeds every 6 h up to hour 72. Over this time course, the embryonic leaves exhibit transcripts of 30,255 genes at a level that can be regarded as "expressed." In dry seeds, ?25,500 genes are expressed, showing functional enrichment in transcription, RNA processing, protein synthesis, primary metabolic pathways, and calcium transport. During the 72-h time course, ?13,900 genes, including 590 transcription factor genes, are differentially expressed. Indeed, by 30 h postimbibition, ?2,200 genes expressed in dry seeds are already down-regulated, and ?2,000 are up-regulated. Moreover, the top 1% expressed genes at 54 h or later are very different from those before 30 h, reflecting important developmental and physiological transitions. Interestingly, clusters of genes involved in hormone metabolism, signaling, and responses are differentially expressed at various time points and TF gene expression is also modular and stage specific. Our dataset provides an opportunity for hypothesizing the timing of regulatory actions, particularly in the context of KS development.

SUBMITTER: Liu WY 

PROVIDER: S-EPMC3593892 | biostudies-literature | 2013 Mar

REPOSITORIES: biostudies-literature

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Anatomical and transcriptional dynamics of maize embryonic leaves during seed germination.

Liu Wen-Yu WY   Chang Yao-Ming YM   Chen Sean Chun-Chang SC   Lu Chen-Hua CH   Wu Yeh-Hwa YH   Lu Mei-Yeh Jade MY   Chen Di-Rong DR   Shih Arthur Chun-Chieh AC   Sheue Chiou-Rong CR   Huang Hsuan-Cheng HC   Yu Chun-Ping CP   Lin Hsin-Hung HH   Shiu Shin-Han SH   Ku Maurice Sun-Ben MS   Li Wen-Hsiung WH   Li Wen-Hsiung WH  

Proceedings of the National Academy of Sciences of the United States of America 20130219 10


Our anatomical analysis revealed that a dry maize seed contains four to five embryonic leaves at different developmental stages. Rudimentary kranz structure (KS) is apparent in the first leaf with a substantial density, but its density decreases toward younger leaves. Upon imbibition, leaf expansion occurs rapidly with new KSs initiated from the palisade-like ground meristem cells in the middle of the leaf. In parallel to the anatomical analysis, we obtained the time course transcriptomes for th  ...[more]

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