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Development of a single-cell array for large-scale DNA fluorescence in situ hybridization.

ABSTRACT: DNA fluorescence in situ hybridization (FISH) is a powerful cytogenetic assay, but conventional sample-preparation methods for FISH do not support large-scale high-throughput data acquisition and analysis, which are potentially useful for several biomedical applications. To address this limitation, we have developed a novel FISH sample-preparation method based on generating a centimetre-sized cell array, in which all cells are precisely positioned and separated from their neighbours. This method is simple and capable of patterning nonadherent human cells. We have successfully performed DNA FISH on the single-cell arrays, which facilitates analysis of the FISH results with the FISH-FINDER computer program.

SUBMITTER: Liu Y

PROVIDER: S-EPMC3594524 | biostudies-literature | 2013 Apr

REPOSITORIES: biostudies-literature

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Development of a single-cell array for large-scale DNA fluorescence in situ hybridization.

Liu Yingru Y Kirkland Brett B Shirley James J Wang Zhibin Z Zhang Peipei P Stembridge Jacquelyn J Wong Wilson W Takebayashi Shin-ichiro S Gilbert David M DM Lenhert Steven S Guan Jingjiao J

Lab on a chip 20130401 7

DNA fluorescence in situ hybridization (FISH) is a powerful cytogenetic assay, but conventional sample-preparation methods for FISH do not support large-scale high-throughput data acquisition and analysis, which are potentially useful for several biomedical applications. To address this limitation, we have developed a novel FISH sample-preparation method based on generating a centimetre-sized cell array, in which all cells are precisely positioned and separated from their neighbours. This method ...[more]

PMID: 23370691

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