Ontology highlight
ABSTRACT:
SUBMITTER: Cao D
PROVIDER: S-EPMC8545947 | biostudies-literature | 2021 Oct
REPOSITORIES: biostudies-literature
Cao Dongjian D Wu Sa S Xi Caili C Li Dong D Zhu Kaiheng K Zhang Zhihong Z Gong Hui H Luo Qingming Q Yang Jie J
Communications biology 20211025 1
Fluorescence in situ hybridization (FISH) is a powerful tool to visualize transcripts in fixed cells and tissues. Despite the recent advances in FISH detection methods, it remains challenging to achieve high-level FISH imaging with a simple workflow. Here, we introduce a method to prepare long single-strand DNA concatemers (lssDNAc) through a controllable rolling-circle amplification (CRCA). Prepared lssDNAcs are used to develop AmpFISH workflows. In addition, we present its applications in diff ...[more]