Direct effects of IL-4 on mast cells drive their intestinal expansion and increase susceptibility to anaphylaxis in a murine model of food allergy.
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ABSTRACT: Interleukin (IL)-4 has critical roles in allergic disorders, including food hypersensitivity. The direct effects of the cytokine on the survival and function of mast cells, the key effectors of food anaphylaxis, have not been established. In this study, we demonstrate that IL-4 induces a marked intestinal mastocytosis in mice. This phenotype is reproduced in animals expressing Il4r?F709, an activating variant of the IL-4 receptor ?-chain (IL-4R?). Il4r?F709 mice exhibit enhanced anaphylactic reactions but unaltered physiological responses to vasoactive mediators. IL-4 induces Bcl-2 and Bcl-X(L) and enhances survival and stimulates proliferation in cultured bone marrow-derived mast cells (BMMC). These effects are STAT6 (signal transducer and activator of transcription factor 6)-dependent and are amplified in Il4r?F709 BMMC. In competitive bone marrow chimeras, Il4r?F709 mast cells display a substantial competitive advantage over wild-type mast cells, which, in turn, prevail over IL-4R??/? mast cells in populating the intestine, establishing a cell-intrinsic effect of IL-4 in intestinal mast cell homeostasis. Our results demonstrate that IL-4-signaling is a key determinant of mast cell expansion in food allergy.
SUBMITTER: Burton OT
PROVIDER: S-EPMC3600405 | biostudies-literature | 2013 Jul
REPOSITORIES: biostudies-literature
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