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Quantitative optical trapping on single organelles in cell extract.

ABSTRACT: We have developed an optical trapping method to precisely measure the force generated by motor proteins on single organelles of unknown size in cell extract. This approach, termed VMatch, permits the functional interrogation of native motor complexes. We apply VMatch to measure the force, number and activity of kinesin-1 on motile lipid droplets isolated from the liver of normally fed and food-deprived rats.

SUBMITTER: Barak P

PROVIDER: S-EPMC3605797 | biostudies-literature | 2013 Jan

REPOSITORIES: biostudies-literature

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Quantitative optical trapping on single organelles in cell extract.

Barak Pradeep P Rai Ashim A Rai Priyanka P Mallik Roop R

Nature methods 20121216 1

We have developed an optical trapping method to precisely measure the force generated by motor proteins on single organelles of unknown size in cell extract. This approach, termed VMatch, permits the functional interrogation of native motor complexes. We apply VMatch to measure the force, number and activity of kinesin-1 on motile lipid droplets isolated from the liver of normally fed and food-deprived rats. ...[more]

PMID: 23241632

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