Ontology highlight
ABSTRACT:
SUBMITTER: Lang MJ
PROVIDER: S-EPMC1483847 | biostudies-literature | 2004 Nov
REPOSITORIES: biostudies-literature
Nature methods 20041021 2
We constructed a microscope-based instrument capable of simultaneous, spatially coincident optical trapping and single-molecule fluorescence. The capabilities of this apparatus were demonstrated by studying the force-induced strand separation of a dye-labeled, 15-base-pair region of double-stranded DNA (dsDNA), with force applied either parallel ('unzipping' mode) or perpendicular ('shearing' mode) to the long axis of the region. Mechanical transitions corresponding to DNA hybrid rupture occurre ...[more]