Conditional genome engineering in Toxoplasma gondii uncovers alternative invasion mechanisms.
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ABSTRACT:
SUBMITTER: Andenmatten N
PROVIDER: S-EPMC3605914 | biostudies-literature | 2013 Feb
REPOSITORIES: biostudies-literature
Publications
Conditional genome engineering in Toxoplasma gondii uncovers alternative invasion mechanisms.
Andenmatten Nicole N Egarter Saskia S Jackson Allison J AJ Jullien Nicolas N Herman Jean-Paul JP Meissner Markus M
Nature methods 20121223 2
We established a conditional site-specific recombination system based on dimerizable Cre recombinase-mediated recombination in the apicomplexan parasite Toxoplasma gondii. Using a new single-vector strategy that allows ligand-dependent, efficient removal of a gene of interest, we generated three knockouts of apicomplexan genes considered essential for host-cell invasion. Our findings uncovered the existence of an alternative invasion pathway in apicomplexan parasites. ...[more]
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