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Conditional genome engineering in Toxoplasma gondii uncovers alternative invasion mechanisms.


ABSTRACT: We established a conditional site-specific recombination system based on dimerizable Cre recombinase-mediated recombination in the apicomplexan parasite Toxoplasma gondii. Using a new single-vector strategy that allows ligand-dependent, efficient removal of a gene of interest, we generated three knockouts of apicomplexan genes considered essential for host-cell invasion. Our findings uncovered the existence of an alternative invasion pathway in apicomplexan parasites.

SUBMITTER: Andenmatten N 

PROVIDER: S-EPMC3605914 | biostudies-literature | 2013 Feb

REPOSITORIES: biostudies-literature

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Conditional genome engineering in Toxoplasma gondii uncovers alternative invasion mechanisms.

Andenmatten Nicole N   Egarter Saskia S   Jackson Allison J AJ   Jullien Nicolas N   Herman Jean-Paul JP   Meissner Markus M  

Nature methods 20121223 2


We established a conditional site-specific recombination system based on dimerizable Cre recombinase-mediated recombination in the apicomplexan parasite Toxoplasma gondii. Using a new single-vector strategy that allows ligand-dependent, efficient removal of a gene of interest, we generated three knockouts of apicomplexan genes considered essential for host-cell invasion. Our findings uncovered the existence of an alternative invasion pathway in apicomplexan parasites. ...[more]

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