Ontology highlight
ABSTRACT:
SUBMITTER: Pieperhoff MS
PROVIDER: S-EPMC4474610 | biostudies-literature | 2015
REPOSITORIES: biostudies-literature
Pieperhoff Manuela S MS Pall Gurman S GS Jiménez-Ruiz Elena E Das Sujaan S Melatti Carmen C Gow Matthew M Wong Eleanor H EH Heng Joanne J Müller Sylke S Blackman Michael J MJ Meissner Markus M
PloS one 20150619 6
The functional characterisation of essential genes in apicomplexan parasites, such as Toxoplasma gondii or Plasmodium falciparum, relies on conditional mutagenesis systems. Here we present a novel strategy based on U1 snRNP-mediated gene silencing. U1 snRNP is critical in pre-mRNA splicing by defining the exon-intron boundaries. When a U1 recognition site is placed into the 3'-terminal exon or adjacent to the termination codon, pre-mRNA is cleaved at the 3'-end and degraded, leading to an effici ...[more]