Ontology highlight
ABSTRACT:
SUBMITTER: Lee SC
PROVIDER: S-EPMC3612649 | biostudies-literature | 2013 Mar
REPOSITORIES: biostudies-literature
Lee Sung Chang SC Bennett Brad C BC Hong Wen-Xu WX Fu Yu Y Baker Kent A KA Marcoux Julien J Robinson Carol V CV Ward Andrew B AB Halpert James R JR Stevens Raymond C RC Stout Charles David CD Yeager Mark J MJ Zhang Qinghai Q
Proceedings of the National Academy of Sciences of the United States of America 20130311 13
Amphiphile selection is a critical step for structural studies of membrane proteins (MPs). We have developed a family of steroid-based facial amphiphiles (FAs) that are structurally distinct from conventional detergents and previously developed FAs. The unique FAs stabilize MPs and form relatively small protein-detergent complexes (PDCs), a property considered favorable for MP crystallization. We attempted to crystallize several MPs belonging to different protein families, including the human ga ...[more]