Ontology highlight
ABSTRACT:
SUBMITTER: Dunn BJ
PROVIDER: S-EPMC3612939 | biostudies-literature | 2013 Mar
REPOSITORIES: biostudies-literature
Dunn Briana J BJ Cane David E DE Khosla Chaitan C
Biochemistry 20130305 11
Acyltransferase (AT) domains of modular polyketide synthases exercise tight control over the choice of α-carboxyacyl-CoA substrates, but the mechanistic basis for this specificity is unknown. We show that whereas the specificity for the electrophilic malonyl or methylmalonyl component is primarily expressed in the first half-reaction (formation of the acyl-enzyme intermediate), the second half-reaction shows comparable specificity for the acyl carrier protein that carries the nucleophilic pantet ...[more]