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The scaffold protein EPG-7 links cargo-receptor complexes with the autophagic assembly machinery.

ABSTRACT: The mechanism by which protein aggregates are selectively degraded by autophagy is poorly understood. Previous studies show that a family of Atg8-interacting proteins function as receptors linking specific cargoes to the autophagic machinery. Here we demonstrate that during Caenorhabditis elegans embryogenesis, epg-7 functions as a scaffold protein mediating autophagic degradation of several protein aggregates, including aggregates of the p62 homologue SQST-1, but has little effect on other autophagy-regulated processes. EPG-7 self-oligomerizes and is degraded by autophagy independently of SQST-1. SQST-1 directly interacts with EPG-7 and colocalizes with EPG-7 aggregates in autophagy mutants. Mutations in epg-7 impair association of SQST-1 aggregates with LGG-1/Atg8 puncta. EPG-7 interacts with multiple ATG proteins and colocalizes with ATG-9 puncta in various autophagy mutants. Unlike core autophagy genes, epg-7 is dispensable for starvation-induced autophagic degradation of substrate aggregates. Our results indicate that under physiological conditions a scaffold protein endows cargo specificity and also elevates degradation efficiency by linking the cargo-receptor complex with the autophagic machinery.

SUBMITTER: Lin L

PROVIDER: S-EPMC3613692 | biostudies-literature | 2013 Apr

REPOSITORIES: biostudies-literature

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The scaffold protein EPG-7 links cargo-receptor complexes with the autophagic assembly machinery.

Lin Long L Yang Peiguo P Huang Xinxin X Zhang Hui H Lu Qun Q Zhang Hong H

The Journal of cell biology 20130325 1

The mechanism by which protein aggregates are selectively degraded by autophagy is poorly understood. Previous studies show that a family of Atg8-interacting proteins function as receptors linking specific cargoes to the autophagic machinery. Here we demonstrate that during Caenorhabditis elegans embryogenesis, epg-7 functions as a scaffold protein mediating autophagic degradation of several protein aggregates, including aggregates of the p62 homologue SQST-1, but has little effect on other auto ...[more]

PMID: 23530068

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Dataset Information

The scaffold protein EPG-7 links cargo-receptor complexes with the autophagic assembly machinery.

Publications

The scaffold protein EPG-7 links cargo-receptor complexes with the autophagic assembly machinery.

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