?5i subunit deficiency of the immunoproteasome leads to reduced Th2 response in OVA induced acute asthma.

ABSTRACT: The immunoproteasome subunit ?5i has been shown to play an important role in Th1/Th17 driven models of colitis and arthritis. However, the function of ?5i in Th2 dependent diseases remains enigmatic. To study the role of ?5i in Th2-driven pathology, ?5i knockout (KO) and control mice were tested in different models of experimental allergic asthma. ?5i-deficient mice showed reduced OVA/Alum- and subcutaneous/OVA-induced acute asthma with decreased eosinophilia in the bronchoalveolar lavage (BAL), low OVA-specific IgG1 and reduced local and systemic Th2 cytokines. While Th2 cells in the lungs were reduced, Tregs and Th1 cells were not affected. Attenuated asthma in ?5i KO mice could not be attributed to defects in OVA uptake or maturation of dendritic cells in the lung. Surprisingly, ?5i deficient mice developed HDM asthma which was comparable to control mice. Here, we present novel evidence for the requirement of the ?5i immunosubunit to generate a strong Th2 response during OVA- but not HDM-induced acute asthma. The unexpected role of ?5i in OVA asthma remains to be clarified.

SUBMITTER: Volkov A 

PROVIDER: S-EPMC3617144 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature