Ontology highlight
ABSTRACT:
SUBMITTER: Moreno D
PROVIDER: S-EPMC3619943 | biostudies-literature | 2013
REPOSITORIES: biostudies-literature
Moreno Daniel D Neller Joachim J Kestler Hans A HA Kraus Johann J Dünkler Alexander A Johnsson Nils N
Molecular systems biology 20130101
We introduce a fluorescent reporter for monitoring protein-protein interactions in living cells. The method is based on the Split-Ubiquitin method and uses the ratio of two auto-fluorescent reporter proteins as signal for interaction (SPLIFF). The mating of two haploid yeast cells initiates the analysis and the interactions are followed online by two-channel time-lapse microscopy of the diploid cells during their first cell cycle. Using this approach we could with high spatio-temporal resolution ...[more]