Ontology highlight
ABSTRACT:
SUBMITTER: Drepper T
PROVIDER: S-EPMC2935039 | biostudies-literature | 2010 Sep
REPOSITORIES: biostudies-literature
Drepper Thomas T Huber Robert R Heck Achim A Circolone Franco F Hillmer Anne-Kathrin AK Büchs Jochen J Jaeger Karl-Erich KE
Applied and environmental microbiology 20100702 17
Fluorescent proteins of the green fluorescent protein (GFP) family are commonly used as reporter proteins for quantitative analysis of complex biological processes in living microorganisms. Here we demonstrate that the fluorescence signal intensity of GFP-like proteins is affected under oxygen limitation and therefore does not reflect the amount of reporter protein in Escherichia coli batch cultures. Instead, flavin mononucleotide (FMN)-binding fluorescent proteins (FbFPs) are suitable for quant ...[more]