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Label-free polypeptide-based enzyme detection using a graphene-nanoparticle hybrid sensor.

ABSTRACT: A graphene-nanoparticle (NP) hybrid biosensor that utilizes an electrical hysteresis change to detect the enzymatic activity and concentration of Carboxypeptidase B was developed. The results indicate that the novel graphene-NP hybrid biosensor, utilizing electrical hysteresis, has the ability to detect concentrations of targeted enzyme on the micromolar scale. Furthermore, to the knowledge of the authors, this is the first demonstration of a graphene-based biosensor that utilizes a hysteresis change resulting from metallic NPs assembled on a graphene surface.

SUBMITTER: Myung S

PROVIDER: S-EPMC3626444 | biostudies-literature | 2012 Nov

REPOSITORIES: biostudies-literature

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Label-free polypeptide-based enzyme detection using a graphene-nanoparticle hybrid sensor.

Myung Sung S Yin Perry T PT Kim Cheoljin C Park Jaesung J Solanki Aniruddh A Reyes Pavel Ivanoff PI Lu Yicheng Y Kim Kwang S KS Lee Ki-Bum KB

Advanced materials (Deerfield Beach, Fla.) 20120907 45

A graphene-nanoparticle (NP) hybrid biosensor that utilizes an electrical hysteresis change to detect the enzymatic activity and concentration of Carboxypeptidase B was developed. The results indicate that the novel graphene-NP hybrid biosensor, utilizing electrical hysteresis, has the ability to detect concentrations of targeted enzyme on the micromolar scale. Furthermore, to the knowledge of the authors, this is the first demonstration of a graphene-based biosensor that utilizes a hysteresis c ...[more]

PMID: 22961629

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