Ontology highlight
ABSTRACT:
SUBMITTER: Yokomizo T
PROVIDER: S-EPMC3629302 | biostudies-literature | 2012 Feb
REPOSITORIES: biostudies-literature
Yokomizo Tomomasa T Yamada-Inagawa Tomoko T Yzaguirre Amanda D AD Chen Michael J MJ Speck Nancy A NA Dzierzak Elaine E
Nature protocols 20120209 3
We describe a three-dimensional (3D) confocal imaging technique to characterize and enumerate rare, newly emerging hematopoietic cells located within the vasculature of whole-mount preparations of mouse embryos. However, the methodology is broadly applicable for examining the development and 3D architecture of other tissues. Previously, direct whole-mount imaging has been limited to external tissue layers owing to poor laser penetration of dense, opaque tissue. Our whole-embryo imaging method en ...[more]