Ontology highlight
ABSTRACT:
SUBMITTER: Sato M
PROVIDER: S-EPMC8744978 | biostudies-literature | 2021 Dec
REPOSITORIES: biostudies-literature
Sato Moeko M Akashi Hiroko H Sakamoto Yuki Y Matsunaga Sachihiro S Tsuji Hiroyuki H
International journal of molecular sciences 20211221 1
The three-dimensional (3D) arrangement of cells in tissues provides an anatomical basis for analyzing physiological and biochemical aspects of plant and animal cellular development and function. In this study, we established a protocol for tissue clearing and 3D imaging in rice. Our protocol is based on three improvements: clearing with iTOMEI (clearing solution suitable for plants), developing microscopic conditions in which the Z step is optimized for 3D reconstruction, and optimizing cell-wal ...[more]