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A universal vector for high-efficiency multi-fragment recombineering of BACs and knock-in constructs.

ABSTRACT: There is an increasing need for more efficient generation of transgenic constructs. Here we present a universal multi-site Gateway vector for use in recombineering reactions. Using transgenic mouse models, we show its use for the generation of BAC transgenics and targeting vectors. The modular nature of the vector allows for rapid modification of constructs to generate different versions of the same construct. As such it will help streamline the generation of series of related transgenic models.

SUBMITTER: Dolt KS

PROVIDER: S-EPMC3639282 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature

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A universal vector for high-efficiency multi-fragment recombineering of BACs and knock-in constructs.

Dolt Karamjit Singh KS Lawrence Melanie L ML Miller-Hodges Eve E Slight Joan J Thornburn Anna A Devenney Paul S PS Hohenstein Peter P

PloS one 20130429 4

There is an increasing need for more efficient generation of transgenic constructs. Here we present a universal multi-site Gateway vector for use in recombineering reactions. Using transgenic mouse models, we show its use for the generation of BAC transgenics and targeting vectors. The modular nature of the vector allows for rapid modification of constructs to generate different versions of the same construct. As such it will help streamline the generation of series of related transgenic models. ...[more]

PMID: 23637962

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