Ontology highlight
ABSTRACT:
SUBMITTER: Zhao J
PROVIDER: S-EPMC6511857 | biostudies-literature | 2019 May
REPOSITORIES: biostudies-literature
Zhao Jia J Pokhilko Alexandra A Ebenhöh Oliver O Rosser Susan J SJ Colloms Sean D SD
Nucleic acids research 20190501 9
A device that counts and records the number of events experienced by an individual cell could have many uses in experimental biology and biotechnology. Here, we report a DNA-based 'latch' that switches between two states upon each exposure to a repeated stimulus. The key component of the latch is a DNA segment whose orientation is inverted by the actions of ϕC31 integrase and its recombination directionality factor (RDF). Integrase expression is regulated by an external input, while RDF expressi ...[more]