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Phosphorylation of mitophagy and pexophagy receptors coordinates their interaction with Atg8 and Atg11.

ABSTRACT: The selective autophagy receptors Atg19 and Atg32 interact with two proteins of the core autophagic machinery: the scaffold protein Atg11 and the ubiquitin-like protein Atg8. We found that the Pichia pastoris pexophagy receptor, Atg30, also interacts with Atg8. Both Atg30 and Atg32 interactions are regulated by phosphorylation close to Atg8-interaction motifs. Extending this finding to Saccharomyces cerevisiae, we confirmed phosphoregulation for the mitophagy and pexophagy receptors, Atg32 and Atg36. Each Atg30 molecule must interact with both Atg8 and Atg11 for full functionality, and these interactions occur independently and not simultaneously, but rather in random order. We present a common model for the phosphoregulation of selective autophagy receptors.

SUBMITTER: Farre JC

PROVIDER: S-EPMC3642380 | biostudies-literature | 2013 May

REPOSITORIES: biostudies-literature

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Phosphorylation of mitophagy and pexophagy receptors coordinates their interaction with Atg8 and Atg11.

Farré Jean-Claude JC Burkenroad Aaron A Burnett Sarah F SF Subramani Suresh S

EMBO reports 20130405 5

The selective autophagy receptors Atg19 and Atg32 interact with two proteins of the core autophagic machinery: the scaffold protein Atg11 and the ubiquitin-like protein Atg8. We found that the Pichia pastoris pexophagy receptor, Atg30, also interacts with Atg8. Both Atg30 and Atg32 interactions are regulated by phosphorylation close to Atg8-interaction motifs. Extending this finding to Saccharomyces cerevisiae, we confirmed phosphoregulation for the mitophagy and pexophagy receptors, Atg32 and A ...[more]

PMID: 23559066

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