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Sensitive and specific single-molecule sequencing of 5-hydroxymethylcytosine.

ABSTRACT: We describe strand-specific, base-resolution detection of 5-hydroxymethylcytosine (5-hmC) in genomic DNA with single-molecule sensitivity, combining a bioorthogonal, selective chemical labeling method of 5-hmC with single-molecule, real-time (SMRT) DNA sequencing. The chemical labeling not only allows affinity enrichment of 5-hmC-containing DNA fragments but also enhances the kinetic signal of 5-hmC during SMRT sequencing. We applied the approach to sequence 5-hmC in a genomic DNA sample with high confidence.

SUBMITTER: Song CX

PROVIDER: S-EPMC3646335 | biostudies-literature | 2011 Nov

REPOSITORIES: biostudies-literature

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Sensitive and specific single-molecule sequencing of 5-hydroxymethylcytosine.

Song Chun-Xiao CX Clark Tyson A TA Lu Xing-Yu XY Kislyuk Andrey A Dai Qing Q Turner Stephen W SW He Chuan C Korlach Jonas J

Nature methods 20111120 1

We describe strand-specific, base-resolution detection of 5-hydroxymethylcytosine (5-hmC) in genomic DNA with single-molecule sensitivity, combining a bioorthogonal, selective chemical labeling method of 5-hmC with single-molecule, real-time (SMRT) DNA sequencing. The chemical labeling not only allows affinity enrichment of 5-hmC-containing DNA fragments but also enhances the kinetic signal of 5-hmC during SMRT sequencing. We applied the approach to sequence 5-hmC in a genomic DNA sample with hi ...[more]

PMID: 22101853

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