Ontology highlight
ABSTRACT:
SUBMITTER: Deindl S
PROVIDER: S-EPMC3647478 | biostudies-literature | 2013 Jan
REPOSITORIES: biostudies-literature
Deindl Sebastian S Hwang William L WL Hota Swetansu K SK Blosser Timothy R TR Prasad Punit P Bartholomew Blaine B Zhuang Xiaowei X
Cell 20130101 3
ISWI-family enzymes remodel chromatin by sliding nucleosomes along DNA, but the nucleosome translocation mechanism remains unclear. Here we use single-molecule FRET to probe nucleosome translocation by ISWI-family remodelers. Distinct ISWI-family members translocate nucleosomes with a similar stepping pattern maintained by the catalytic subunit of the enzyme. Nucleosome remodeling begins with a 7 bp step of DNA translocation followed by 3 bp subsequent steps toward the exit side of nucleosomes. ...[more]