Ontology highlight
ABSTRACT:
SUBMITTER: Papaparaskevas J
PROVIDER: S-EPMC3647933 | biostudies-literature | 2013 May
REPOSITORIES: biostudies-literature
Papaparaskevas Joseph J Mela Victoria V Houhoula Dimitra P DP Pantazatou Angeliki A Petrikkos Georgios L GL Tsakris Athanassios A
Journal of clinical microbiology 20130227 5
A conventional PCR and a real-time PCR for detecting Bacteroides fragilis were evaluated against clinical specimens. Analytical sensitivities were 100 and 40 fg of DNA, respectively. Detection limits were 100 and 10 CFU/ml, respectively. A total of six culture-negative specimens were positive by PCR. Altering the gold standard from "positive culture" to "positive culture or both PCR assays positive" resulted in sensitivities of 91.7% and 100%, respectively, and in specificities of 100% and 98.6% ...[more]