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Comparative evaluation of conventional and real-time PCR assays for detecting Bacteroides fragilis in clinical samples.


ABSTRACT: A conventional PCR and a real-time PCR for detecting Bacteroides fragilis were evaluated against clinical specimens. Analytical sensitivities were 100 and 40 fg of DNA, respectively. Detection limits were 100 and 10 CFU/ml, respectively. A total of six culture-negative specimens were positive by PCR. Altering the gold standard from "positive culture" to "positive culture or both PCR assays positive" resulted in sensitivities of 91.7% and 100%, respectively, and in specificities of 100% and 98.6%, respectively.

SUBMITTER: Papaparaskevas J 

PROVIDER: S-EPMC3647933 | biostudies-literature | 2013 May

REPOSITORIES: biostudies-literature

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Comparative evaluation of conventional and real-time PCR assays for detecting Bacteroides fragilis in clinical samples.

Papaparaskevas Joseph J   Mela Victoria V   Houhoula Dimitra P DP   Pantazatou Angeliki A   Petrikkos Georgios L GL   Tsakris Athanassios A  

Journal of clinical microbiology 20130227 5


A conventional PCR and a real-time PCR for detecting Bacteroides fragilis were evaluated against clinical specimens. Analytical sensitivities were 100 and 40 fg of DNA, respectively. Detection limits were 100 and 10 CFU/ml, respectively. A total of six culture-negative specimens were positive by PCR. Altering the gold standard from "positive culture" to "positive culture or both PCR assays positive" resulted in sensitivities of 91.7% and 100%, respectively, and in specificities of 100% and 98.6%  ...[more]

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