Reconstitution of EPA and DHA biosynthesis in arabidopsis: iterative metabolic engineering for the synthesis of n-3 LC-PUFAs in transgenic plants.
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ABSTRACT:
SUBMITTER: Ruiz-Lopez N
PROVIDER: S-EPMC3650579 | biostudies-literature | 2013 May
REPOSITORIES: biostudies-literature
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Reconstitution of EPA and DHA biosynthesis in arabidopsis: iterative metabolic engineering for the synthesis of n-3 LC-PUFAs in transgenic plants.
Ruiz-Lopez Noemi N Haslam Richard P RP Usher Sarah L SL Napier Johnathan A JA Sayanova Olga O
Metabolic engineering 20130314
An iterative approach to optimising the accumulation of non-native long chain polyunsaturated fatty acids in transgenic plants was undertaken in Arabidopsis thaliana. The contribution of a number of different transgene enzyme activities was systematically determined, as was the contribution of endogenous fatty acid metabolism. Successive iterations were informed by lipidomic analysis of neutral, polar and acyl-CoA pools. This approach allowed for a four-fold improvement on levels previously repo ...[more]
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