Unknown

Dataset Information

0

Nucleotide-resolution DNA double-strand break mapping by next-generation sequencing.


ABSTRACT: We present a genome-wide approach to map DNA double-strand breaks (DSBs) at nucleotide resolution by a method we termed BLESS (direct in situ breaks labeling, enrichment on streptavidin and next-generation sequencing). We validated and tested BLESS using human and mouse cells and different DSBs-inducing agents and sequencing platforms. BLESS was able to detect telomere ends, Sce endonuclease-induced DSBs and complex genome-wide DSB landscapes. As a proof of principle, we characterized the genomic landscape of sensitivity to replication stress in human cells, and we identified >2,000 nonuniformly distributed aphidicolin-sensitive regions (ASRs) overrepresented in genes and enriched in satellite repeats. ASRs were also enriched in regions rearranged in human cancers, with many cancer-associated genes exhibiting high sensitivity to replication stress. Our method is suitable for genome-wide mapping of DSBs in various cells and experimental conditions, with a specificity and resolution unachievable by current techniques.

SUBMITTER: Crosetto N 

PROVIDER: S-EPMC3651036 | biostudies-literature | 2013 Apr

REPOSITORIES: biostudies-literature

altmetric image

Publications


We present a genome-wide approach to map DNA double-strand breaks (DSBs) at nucleotide resolution by a method we termed BLESS (direct in situ breaks labeling, enrichment on streptavidin and next-generation sequencing). We validated and tested BLESS using human and mouse cells and different DSBs-inducing agents and sequencing platforms. BLESS was able to detect telomere ends, Sce endonuclease-induced DSBs and complex genome-wide DSB landscapes. As a proof of principle, we characterized the genomi  ...[more]

Similar Datasets

| S-EPMC6061839 | biostudies-literature
| S-EPMC5021761 | biostudies-literature
| S-EPMC9074467 | biostudies-literature
| S-EPMC7408924 | biostudies-literature
| S-EPMC5120557 | biostudies-literature
| S-EPMC5320478 | biostudies-literature
| S-EPMC6294500 | biostudies-literature
| S-EPMC2725361 | biostudies-literature
| S-EPMC7849381 | biostudies-literature
| S-EPMC5821106 | biostudies-literature