Ontology highlight
ABSTRACT:
SUBMITTER: de Paz AM
PROVIDER: S-EPMC6061839 | biostudies-literature | 2018 Jul
REPOSITORIES: biostudies-literature
de Paz Alexandra M AM Cybulski Thaddeus R TR Marblestone Adam H AH Zamft Bradley M BM Church George M GM Boyden Edward S ES Kording Konrad P KP Tyo Keith E J KEJ
Nucleic acids research 20180701 13
DNA polymerase fidelity is affected by both intrinsic properties and environmental conditions. Current strategies for measuring DNA polymerase error rate in vitro are constrained by low error subtype sensitivity, poor scalability, and lack of flexibility in types of sequence contexts that can be tested. We have developed the Magnification via Nucleotide Imbalance Fidelity (MagNIFi) assay, a scalable next-generation sequencing assay that uses a biased deoxynucleotide pool to quantitatively shift ...[more]