Ontology highlight
ABSTRACT:
SUBMITTER: Gantt RW
PROVIDER: S-EPMC3651490 | biostudies-literature | 2013 May
REPOSITORIES: biostudies-literature
Proceedings of the National Academy of Sciences of the United States of America 20130422 19
We described the integration of the general reversibility of glycosyltransferase-catalyzed reactions, artificial glycosyl donors, and a high throughput colorimetric screen to enable the engineering of glycosyltransferases for combinatorial sugar nucleotide synthesis. The best engineered catalyst from this study, the OleD Loki variant, contained the mutations P67T/I112P/T113M/S132F/A242I compared with the OleD wild-type sequence. Evaluated against the parental sequence OleD TDP16 variant used for ...[more]