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A simple strategy for glycosyltransferase-catalyzed aminosugar nucleotide synthesis.

ABSTRACT: A set of 2-chloro-4-nitrophenyl glucosamino-/xylosaminosides were synthesized and assessed as potential substrates in the context of glycosyltransferase-catalyzed formation of the corresponding UDP/TDP-?-D-glucosamino-/xylosaminosugars and in single-vessel model transglycosylation reactions. This study highlights a robust platform for aminosugar nucleotide synthesis and reveals OleD Loki to be a proficient catalyst for U/TDP-aminosugar synthesis and utilization

SUBMITTER: Zhang J

PROVIDER: S-EPMC4051237 | biostudies-literature | 2014 Mar

REPOSITORIES: biostudies-literature

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A simple strategy for glycosyltransferase-catalyzed aminosugar nucleotide synthesis.

Zhang Jianjun J Singh Shanteri S Hughes Ryan R RR Zhou Maoquan M Sunkara Manjula M Morris Andrew J AJ Thorson Jon S JS

Chembiochem : a European journal of chemical biology 20140301 5

A set of 2-chloro-4-nitrophenyl glucosamino-/xylosaminosides were synthesized and assessed as potential substrates in the context of glycosyltransferase-catalyzed formation of the corresponding UDP/TDP-α-D-glucosamino-/xylosaminosugars and in single-vessel model transglycosylation reactions. This study highlights a robust platform for aminosugar nucleotide synthesis and reveals OleD Loki to be a proficient catalyst for U/TDP-aminosugar synthesis and utilization ...[more]

PMID: 24677528

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