Defining ELISpot cut-offs from unreplicated test and control wells.
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ABSTRACT:
SUBMITTER: Alexander N
PROVIDER: S-EPMC3657161 | biostudies-literature | 2013 Jun
REPOSITORIES: biostudies-literature
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Defining ELISpot cut-offs from unreplicated test and control wells.
Alexander Neal N Fox Annette A Lien Vu Thi Kim VT Dong Tao T Lee Laurel Yong-Hwa LY Hang Nguyen Le Khanh Nle K Mai Le Quynh le Q Horby Peter P
Journal of immunological methods 20130307 1-2
In the absence of replication of wells, empirical criteria for enzyme-linked immunospot (ELISpot) positivity use fixed differences or ratios between spot forming units (SFU) counts between test and control. We propose an alternative approach which first identifies the optimally variance-stabilizing transformation of the SFU counts, based on the Bland-Altman plot of the test and control wells. The second step is to derive a positivity threshold from the difference in between-plate distribution fu ...[more]
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