Ontology highlight
ABSTRACT:
SUBMITTER: Hong H
PROVIDER: S-EPMC3659600 | biostudies-literature | 2012 Oct
REPOSITORIES: biostudies-literature
Hong Hao H Cui Chang-Hao CH Kim Jin-Kwang JK Jin Feng-Xie FX Kim Sun-Chang SC Im Wan-Taek WT
Journal of ginseng research 20121001 4
This study focused on the enzymatic biotransformation of the major ginsenoside Rb1 into Rd for the mass production of minor ginsenosides using a novel recombinant β-glucosidase from Flavobacterium johnsoniae. The gene (bglF3) consisting of 2,235 bp (744 amino acid residues) was cloned and the recombinant enzyme overexpressed in Escherichia coli BL21(DE3) was characterized. This enzyme could transform ginsenoside Rb1 and gypenoside XVII to the ginsenosides Rd and F2, respectively. The glutathione ...[more]