Mapping single-cell-substrate interactions by surface plasmon resonance microscopy.
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ABSTRACT:
SUBMITTER: Wang W
PROVIDER: S-EPMC3660850 | biostudies-literature | 2012 Sep
REPOSITORIES: biostudies-literature
Publications
Mapping single-cell-substrate interactions by surface plasmon resonance microscopy.
Wang Wei W Wang Shaopeng S Liu Qiang Q Wu Jie J Tao Nongjian N
Langmuir : the ACS journal of surfaces and colloids 20120904 37
We report the imaging of the cell-substrate adhesion of a single cell with subcellular spatial resolution. Osmotic pressure was introduced to provide a controllable mechanical stimulation to the cell attached to a substrate, and high-resolution surface plasmon resonance microscopy was used to map the response of the cell, from which local cell-substrate adhesion was determined. In addition to high spatial resolution, the approach is noninvasive and fast and allows for the continuous mapping of c ...[more]
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