Ontology highlight
ABSTRACT:
SUBMITTER: Zhang Y
PROVIDER: S-EPMC3661022 | biostudies-literature | 2012 Apr
REPOSITORIES: biostudies-literature
Zhang Yun Y Cui Weidong W Zhang Hao H Dewald Howard D HD Chen Hao H
Analytical chemistry 20120404 8
Covalent disulfide bond linkage in a protein represents an important challenge for mass spectrometry (MS)-based top-down protein structure analysis as it reduces the backbone cleavage efficiency for MS/MS dissociation. This study presents a strategy for solving this critical issue via integrating electrochemistry (EC) online with a top-down MS approach. In this approach, proteins undergo electrolytic reduction in an electrochemical cell to break disulfide bonds and then undergo online ionization ...[more]