Dataset Information

Novel insights into the bovine polled phenotype and horn ontogenesis in Bovidae.

ABSTRACT: Despite massive research efforts, the molecular etiology of bovine polledness and the developmental pathways involved in horn ontogenesis are still poorly understood. In a recent article, we provided evidence for the existence of at least two different alleles at the Polled locus and identified candidate mutations for each of them. None of these mutations was located in known coding or regulatory regions, thus adding to the complexity of understanding the molecular basis of polledness. We confirm previous results here and exhaustively identify the causative mutation for the Celtic allele (PC) and four candidate mutations for the Friesian allele (PF). We describe a previously unreported eyelash-and-eyelid phenotype associated with regular polledness, and present unique histological and gene expression data on bovine horn bud differentiation in fetuses affected by three different horn defect syndromes, as well as in wild-type controls. We propose the ectopic expression of a lincRNA in PC/p horn buds as a probable cause of horn bud agenesis. In addition, we provide evidence for an involvement of OLIG2, FOXL2 and RXFP2 in horn bud differentiation, and draw a first link between bovine, ovine and caprine Polled loci. Our results represent a first and important step in understanding the genetic pathways and key process involved in horn bud differentiation in Bovidae.

SUBMITTER: Allais-Bonnet A

PROVIDER: S-EPMC3661542 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature

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Novel insights into the bovine polled phenotype and horn ontogenesis in Bovidae.

Allais-Bonnet Aurélie A Grohs Cécile C Medugorac Ivica I Krebs Stefan S Djari Anis A Graf Alexander A Fritz Sébastien S Seichter Doris D Baur Aurélia A Russ Ingolf I Bouet Stéphan S Rothammer Sophie S Wahlberg Per P Esquerré Diane D Hoze Chris C Boussaha Mekki M Weiss Bernard B Thépot Dominique D Fouilloux Marie-Noëlle MN Rossignol Marie-Noëlle MN van Marle-Köster Este E Hreiðarsdóttir Gunnfríður Elín GE Barbey Sarah S Dozias Dominique D Cobo Emilie E Reversé Patrick P Catros Olivier O Marchand Jean-Luc JL Soulas Pascal P Roy Pierre P Marquant-Leguienne Brigitte B Le Bourhis Daniel D Clément Laetitia L Salas-Cortes Laura L Venot Eric E Pannetier Maëlle M Phocas Florence F Klopp Christophe C Rocha Dominique D Fouchet Michel M Journaux Laurent L Bernard-Capel Carine C Ponsart Claire C Eggen André A Blum Helmut H Gallard Yves Y Boichard Didier D Pailhoux Eric E Capitan Aurélien A

PloS one 20130522 5

Despite massive research efforts, the molecular etiology of bovine polledness and the developmental pathways involved in horn ontogenesis are still poorly understood. In a recent article, we provided evidence for the existence of at least two different alleles at the Polled locus and identified candidate mutations for each of them. None of these mutations was located in known coding or regulatory regions, thus adding to the complexity of understanding the molecular basis of polledness. We confir ...[more]

PMID: 23717440

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Project description:Interferons are secretory proteins induced in response to specific extracellular stimuli which stimulate intra- and intercellular networks for regulating innate and acquired immunity, resistance to viral infections, and normal and tumor cell survival and death. Type 1 interferons plays a major role in the CD8 T-cell response to viral infection. The genomic analysis carried out here for type I interferons within Bovidae family shows that cattle, bison, water buffalo, goat, and sheep (all Bovidae), have different number of genes of the different subtypes, with a large increase in the numbers, compared to human and mouse genomes. A phylogenetic analysis of the interferon alpha (IFNA) proteins in this group shows that the genes do not follow the evolutionary pattern of the species, but rather a cycle of duplications and deletions in the different species. In this study we also studied the genetic diversity of the bovine interferon alpha A (IFNAA), as an example of the IFNA genes in cattle, sequencing a fragment of the coding sequence in 18 breeds of cattle from Pakistan, Nigeria and USA. Similarity analysis allowed the allocation of sequences into 22 haplotypes. Bhagnari, Brangus, Sokoto Gudali, and White Fulani, had the highest number of haplotypes, while Angus, Hereford and Nari Master had the least. However, when analyzed by the average haplotype count, Angus, Bhagnari, Hereford, Holstein, Muturu showed the highest values, while Cholistani, Lohani, and Nari Master showed the lowest values. Haplotype 4 was found in the highest number of individuals (74), and in 15 breeds. Sequences for yak, bison, and water buffalo, were included within the bovine haplotypes. Medium Joining network showed that the sequences could be divided into 4 groups: one with highly similar haplotypes containing mostly Asian and African breeds, one with almost all of the Bos taurus American breeds, one mid-diverse group with mostly Asian and African sequences, and one group with highly divergent haplotypes with five N'Dama sequences and one from each of White Fulani, Dhanni, Tharparkar, and Bhagnari. The large genetic diversity found in IFNAA could be a very good indication of the genetic variation among the different genes of IFNA and could be an adaptation for these species in response to viral challenges they face.

Dataset Information

Novel insights into the bovine polled phenotype and horn ontogenesis in Bovidae.

Publications

Novel insights into the bovine polled phenotype and horn ontogenesis in Bovidae.

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