Ontology highlight
ABSTRACT:
SUBMITTER: He Y
PROVIDER: S-EPMC3662055 | biostudies-literature | 2012 Feb
REPOSITORIES: biostudies-literature
He Yufan Y Lu Maolin M Cao Jin J Lu H Peter HP
ACS nano 20120201 2
Combining atomic force microscopy and fluorescence resonance energy transfer spectroscopy (AFM-FRET), we have developed a single-molecule AFM-FRET nanoscopy approach capable of effectively pinpointing and mechanically manipulating a targeted dye-labeled single protein in a large sampling area and simultaneously monitoring the conformational changes of the targeted protein by recording single-molecule FRET time trajectories. We have further demonstrated an application of using this nanoscopy on m ...[more]